Review Article  Open Access

World J Mens Health. 2020 Jan;38(1):48-60. English.
Published online Mar 25, 2019.
Copyright © 2020 Korean Society for Sexual Medicine and Andrology
Sex Differences in Gut Microbiota
Yong Sung Kim,1,2 Tatsuya Unno,3,4 Byung-Yong Kim,5 and Mi-Sung Park6
1Digestive Disease Research Institute and Department of Gastroenterology, Wonkwang University School of Medicine, Iksan, Korea.
2Division of Microbiome Research, VOWLIFE R&D Center, Iksan, Korea.
3Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, Korea.
4Faculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju, Korea.
5ChunLab, Inc., Seoul, Korea.
6Institute for Metabolic Disease, School of Medicine, Wonkwang University, Iksan, Korea.

Correspondence to: Yong Sung Kim. Digestive Disease Research Institute and Department of Gastroenterology, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan 54538, Korea. Tel: +82-63-850-6738, Fax: +82-63-850-6799, Email:
Received January 14, 2019; Accepted February 16, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Humans carry numerous symbiotic microorganisms in their body, most of which are present in the gut. Although recent technological advances have produced extensive research data on gut microbiota, there are various confounding factors (e.g., diet, race, medications) to consider. Sex is one of the important variables affecting the gut microbiota, but the association has not yet been sufficiently investigated. Although the results are inconsistent, several animal and human studies have shown sex differences in gut microbiota. Herein, we review these studies to discuss the sex-dependent differences as well as the possible mechanisms involved.

Gastrointestinal tract; Gonadal steroid hormones; Microbiota; Sex differences

Numerous microorganisms exist in many sites of the human body, such as the skin, mouth, and vagina, but most of them are in the gut. The term ‘gut microbiota’ refers to all microorganisms present in the gastrointestinal (GI) tract, being composed mostly of bacteria and some viruses and fungi [1]. Approximately 1,000 to 1,150 bacterial species of bacteria are capable of existing in the human colon, and each individual harbors at least 160 species [1, 2]. Because the gut microbiota maintains homeostasis of the host and has functions in immunity and metabolism, the perturbated microbiota (dysbiosis) is associated with not only GI diseases but also other diseases such as cancers, metabolic diseases, allergies, and immunologic disorders [3, 4]. Recent advances in sequencing technology have resulted in a large quantity of data on gut microbiota being produced. It is known that diverse factors, such as diet, race, age, antibiotics, stress, psychological factors, mother's health, mode of delivery, environmental factor, and exercise, can influence the status of the gut microbiota [5]. Therefore, these factors should be considered when planning a study on the microbiota and interpreting the results. Besides these well-known confounding factors, sex or gender might also be an important factor. However, this factor had been ignored by researchers in spite of its importance. Although the results are inconsistent, animal and human studies have shown sex-related differences in gut microbiota [6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19]. However, some studies showed no such sex difference [9], and the effect of sex on the gut microbiota appears to be less influential than that of other factors [20, 21]. In this review, we discuss the animal and human studies on sex differences in the gut microbiota as well as the possible mechanisms involved.


‘Sex’ refers to the biological classification of a species as based on its reproductive systems and the functions derived from a chromosomal type or hormones. The terms ‘male’ and ‘female’ are used when describing the sex of human participants or other sex-related factors [22]. The term ‘gender’ refers to the cultural attitudes and behaviors associated with stereotypical attitudes regarding a person's sex that shape our conceptions of masculinity and femininity [22]. Researchers often confuse these terms and use them indiscriminately even within one article. Because this review deals with the gut microbiota in animal and human studies, we will use the term ‘sex’.

Despite that sex difference can be an important factor not only in human research but also in preclinical research, its importance has only recently been recognized. Most preclinical animal experiments have used male animals, and even some studies did not report the sex of the animal used [23]. Major granting organizations encourage the use of sex or gender as a biological variable regardless of whether an animal study or a clinical trial is being performed [24]. They also recommend reporting the sex or gender of study participants and the sex of animals as well as the origin of cells. The reason for using sex or gender as a variable is due to the issue of reproducibility of the experiment. In this respect, sex analysis should be performed routinely in studies on the gut microbiota, which is still not actively being done despite that such studies are increasing rapidly.


Unexpectedly, the number of human genes coding for proteins found in Human Genomic Projects is only ~20,000, whereas symbiotic bacteria have approximately 100,000 genes, indicating a significant role for them in human physiology [25]. However, we do not know much about gut microbiota yet. Most of the microbial species identified by the molecular method are not cultured or are unculturable [26, 27]. Similarly, researchers have previously stated that the number of gut microbiota is approximately 1014 cells, which is ten times greater than the number of human body cells. This statement has a long chain of citation originating from a rough estimate of bacteria in fecal contents found in one old literature [28]. A recent study using a new calculation has reported that the number of cells in the gut microbiota are similar to that of human body cells. Interestingly, they showed that the ratio of the numbers of bacterial cells to human cells was different between males and females, being approximately 1.3:1 (38×1012 and 30×1012, respectively) for males and 2.2:1 (44×1012 and 21×1012, respectively) for females [29].

The fetal intestine is supposed to be almost sterile first, then colonized mostly with Lactobacillus species at birth when the fetus passes the mother's vaginal canal, while gut microbiota of babies delivered cesarean section seemed to lack these bacteria. However, the difference in gut microbiota between the birth types seems to be minimized after solid foods are introduced. In addition to the type of birth, the gut microbiota is also influenced by breast-feeding or formula feeding in early life. On the other hand, a recent study reported the presence of bacteria in the placenta or meconium, but it was likely to be the result of contamination [30]. Following the initial colonization of gut bacteria at birth, the species diversity increases and becomes similar to that of an adult when the child is about three years old. The gut microbiota is partly influenced by sex hormones in adolescence and remains stable after that in adulthood. In the elderly of over 70 years of age, the composition of the gut microbiota can be influenced by the changes in intestinal physiological function [31, 32].

The most potent factor affecting the composition of gut microbiota is the diet. At the species level, the gut microbiota can be divided into three enterotypes according to major microbial community profiles namely, Prevotella, Bacteroides, and Ruminococcus [33]. However, the enterotype is different between Western and Eastern studies [34], and several concerns have been raised to link the discrete enterotypes to human diseases [35].

The factors affecting gut microbiota in the lifelong period are summarized in Fig. 1. As the gut microbiota plays an essential role in the priming and development of the host's immune system, these factors are important in maintaining our health, especially in early life. The normal gut microbiota can be temporarily perturbated by external stressors, such as an infection or antibiotics. Moreover, a fundamental change in living environments, such as immigration or a continuous change in dietary habits, can lead to permanent changes in the gut microbiota [36]. The transient perturbation caused by external stress is usually recovered with time; however, if the degree of such perturbation is too great or takes too long to recover, then dysbiosis will occur [37]. When dysbiosis develops, the normal beneficial function of the gut microbiota is lost, and various disorders could ensue.

Fig. 1
Changes in the gut microbiota with time and sex, and the modulatory factors involved. BMI: body mass index.
Click for larger imageDownload as PowerPoint slide


1. Animal studies

Animal studies, mainly in mice, have clearly shown sex-specific differences in the composition of gut microbiota [6, 7, 8, 38, 39, 40]. For example, a study with non-obese diabetic (NOD)/ShiLtJ mice demonstrated that the abundance of Porphyromonadaceae, Veillonellaceae, Kineosporiaceae, Peptococcaceae, Enterobacteriaceae, Lactobacillaceae, Cytophagaceae, Peptostreptococcaceae, and Bacteroidaceae at the family level was higher in the male mice than in the female mice [6].

However, some studies failed to find significant sex effects because of the noise introduced by confounding factors, such as diet, age, and host genetic background [8]. It has been suggested that compared to the sex of an animal, the species and strain of animal have more effect on its gut microbiota composition than its sex does [8, 20, 39].

In a study using two different mouse strains (BALB/c and B6), the males were shown to have a lower microbial diversity and richness than the females, whereas there was no effect of the mouse strain. In contrast, the ratio of Firmicutes/Bacteroidetes was higher in the BALB/c mice, but this ratio was not influenced by sex [39]. In that study, sex and strain explained 11.6% and 26.5% of the variance in microbiota composition, respectively. Sex also influenced the expression profile of genes related to immune functions in the colon in a mouse strain-dependent way [39]. In a study with eight mouse lines from the Collaborative Cross mouse resource, the similarity values were higher between pools of the same line than between pools of the same sex [20].

When the gut microbiota of 89 different inbred mouse strains was analyzed independently [8], clear differences in the gut microbiota composition and diversity were observed between the sexes within each strain, and sex-specific differences were most apparent in the C57BL/6J and C3H/HeJ strains. In a taxonomic analysis, the phyla Actinobacteria and Tenericutes were more abundant in the male mice than in the female mice in the total cohort. At the genus level, Allobaculum, Anaeroplasma, and Erwinia were more abundant in the male mice, whereas SMB53 from family Clostridiaceae and three members of family Lachnospiraceae (Dorea, Coprococcus, and Ruminococcus) were more abundant in the female mice [8]. When the entire population was examined together, both the magnitude and direction of change in the microbiota composition were different between the mouse strains, and no clear differences in patterns between the sexes were observed. These studies suggested that the impact of sex may depend on the host genotype [8].

2. Human studies

Although the importance of the gut microbiota to human health has been of interest over several decades, few studies have addressed the effects of sex on the gut microbiota in the human intestines (Table 1) [10, 11, 12, 13, 14, 15, 16, 17, 18, 19]. Two earlier European multinational studies reported conflicting results for the sex differences in gut microbiota composition [9, 10]. In a 2005 study on 91 northern Europeans from France, Denmark, Germany, the Netherlands, and the United Kingdom, which conducted fluorescent in situ hybridization using 18 phylogenetic probes, there were no significant differences in the colonic microbiota between sexes according to principal component analysis [9]. In a 2006 study conducted by four centers in France, Germany, Italy, and Sweden, a higher level of the Bacteroides-Prevotella group was observed in the males [10]. However, two species-specific probes targeting Bacteroides vulgatus and Bacteroides putredinis showed no differences in their abundance between males and females [10]. In a 2008 study of Chinese family members, which was conducted using group-specific DGGE profiling of Bacteroides spp., a higher abundance of Bacteroides thetaiotaomicron was identified in the males [11]. These earlier studies reported minimal information owing to technical issues and a small number of subjects, but recent studies using advanced techniques and large cohorts have provided more detailed results on the sex differences in gut microbiota.

Table 1
Summary of human studies regarding sex differences in gut microbiota
Click for larger imageClick for full tableDownload as Excel file

In 2014, researchers who analyzed a 16S rRNA gene sequence data set from the Human Microbiome Project (HMP) Consortium simply reported that sex was associated with the community types identified in the stool, where males were three times more likely to have community type D in which fewer Bacteroides and higher Prevotella were observed [12]. In another large cohort study with two independent, extensively phenotyped cohorts: the Belgian Flemish Gut Flora Project (n=1,106) and the Dutch LifeLines-DEEP study (n=1,135), sex has a 10th effect size among 69 factors that were shown to correlate significantly with overall microbiome community variation [41]. Ever since that HMP analysis report, more studies on sex differences in gut microbiota using 16S rRNA gene sequencing have been reported in recent years. In general, the composition of the gut microbiota seems to be different between sexes, where the α-diversity (i.e., Chao and Shannon) appears to be greater in females. However, the results of each study regarding the differences in microbial taxa between sexes are inconsistent.

In a USA study conducted on a mainly White population, the gut microbiota of the females was characterized by a lower abundance of phylum Bacteroidetes [13]. A study in Italy showed that there were no differences in the lumen-associated microbiota, whereas the mucosa-associated microbiota was different between males and females [16]. The mucosa-associated microbiota in females showed a higher abundance of Actinobacteria, Lactobacillales, Streptococcaceae, and Bifidobacterium and less Veillonellaceae and unclassified Clostridia. At the species level, Gemmiger formicilis was associated with the males and Bifidobacterium adolescentis with the females [16].

In a Spanish study, there were no sex differences in the microbiota diversity, overall composition, phylum level, and the Firmicutes/Bacteroidetes ratio when analyzed without considering the body mass index (BMI) [15]. The finer taxonomic analysis showed a higher abundance of Veillonella and Methanobrevibacter in the males and Bilophila in the females. At the species level, the abundance of Bacteroides caccae was higher in the females, whereas that of Bacteroides plebeius and Coprococcus catus was higher in the males [15]. The sex difference in the microbiota was more obvious when analyzed according to the BMI group in this study [15]. In another study of a large cohort (n=1,135) in the Netherlands, sex was associated with 12 microbial species and 43 metabolic pathways, and the females had a higher abundance of Akkermansia muciniphila even after correcting for all confounding factors, such as diet, lifestyle, and medication [19]. Despite these observations, sex explained only 0.5% of the total variation in the gut microbiota in that study [19].

The studies reported in Asia showed different results from those of the Western studies. In one Japanese study, there was no significant difference in the α-diversity between males and females [18]. That study showed significantly higher levels of Prevotella, Megamonas, Fusobacterium, and Megasphaera in the males, and Bifidobacterium, Ruminococcus, and Akkermansia in the females. A Chinese study reported that there were no overall significant taxonomic differences between males and females. However, at the genus level, Ruminococcus was more abundant in the females [17]. Although a sex difference was observed at the genus level, it was also influenced by the BMI.

There is a report that the sex differences in gut microbiota are more evident when enteric infection is present. In analysis of total gut microbiota from patients with enteric infections (Salmonella, Shiga toxin-producing Escherichia coli, Campylobacter, and Shigella) and of their healthy family members, sex had a significant impact on the overall abundance of taxa, with a slightly higher abundance of Bacteroides in the females and Escherichia in the males [14]. In subgroup analysis, this sex difference in the gut microbiota was not observed in healthy individuals, whereas it was apparent in infected individuals with ten times more different microbial features than that in the healthy individuals [14]. Besides the microbial composition and diversity, a recent study showed that sex was correlated with the functional gene richness of the colon [42].


1. Sex hormone and microbiota interaction

To discuss the mechanism of sex difference in gut microbiota, it is natural to question whether sex hormones may play a role. As sex differences in gut microbiota do not appear until puberty, the role of sex hormones in shaping the gut microbiota composition is supported [6, 7].

The α-diversity becomes significantly different between males and females after puberty [6]. The microbiota compositions before and after puberty were not different in the female mice, whereas the composition deviated after puberty in the male mice, suggesting male sex hormones may play an important role in the sex differences in gut microbiota in mice. When the androgen source was removed by castration, the gut microbiota of the castrated male was similar to that of a female mouse rather than a male mouse. Furthermore, testosterone treatment after gonadectomy prevented the significant changes in the gut microbiota composition that were observed in the untreated males [8].

Fecal microbiota transplantation (FMT) experiment showed further evidence of the effect of sex difference on shaping the gut microbiota. Even after transplanting the same specific pathogen-free (SPF) feces from a female into male and female germ-free (GF) mice, the gut microbiota after puberty was distinctly segregated according to the sex of the recipient mice [6]. Similarly, a fecal suspension from a 32-year-old woman was administered to male and female GF rats, whereupon the microbiota clustered according to the sex of the host animal despite the same fecal inoculum [43].

There seems to be a reciprocal interaction between gut microbiota and sex hormones. Whereas the level of 17β-estradiol was not different between GF and SPF NOD mice, the level of testosterone was higher in the GF females than in the SPF females and lower in the GF males than in the SPF males [7]. A subset of glycerophospholipid and sphingolipid metabolites was also different between the SPF male and SPF female mice [7]. When the cecal content of a male mouse was administered to weaning GF female mice, the testosterone levels increased in the recipient GF females mice and persisted during the adult stage, but not in GF female mice that received cecal content from a female mouse. This difference disappeared in old age [7]. These results suggest that the commensal bacteria regulate the production and/or utilization of testosterone and cause a difference in metabolism.

Several studies have also suggested the interaction between estrogen and gut microbiota [44]. Bilateral ovariectomy causes microbial dysbiosis in the mouse [8, 45]. In humans, it has been reported that bilateral ovariectomy is related to an increased abundance of Clostridium bolteae [19]. In male and postmenopausal female, but not premenopausal female, the levels of total urinary estrogen were significantly associated with the richness and α-diversity of intestinal microbiota [46]. The higher that the level of non-ovarian systemic estrogens was, the higher was the abundance of fecal Clostridia, including non-Clostridiales and three genera in the Ruminococcaceae family. Interestingly, soy isoflavones, whose metabolites are structurally similar to estrogen, can significantly alter the structure and composition of the intestinal microbial community in the postmenopausal female by increasing the concentration of Bifidobacterium while suppressing unclassified Clostridiaceae [47]. Although the gut microbiota can be modulated by estrogen, gut microbiota can itself also affect the estrogen level by deconjugating the conjugated estrogen molecules excreted through the bile and reabsorbing it through the enterohepatic circulation [46].

2. Drugs

In contrast to the gut microbiota in experimental animals growing in a well-controlled environment, the gut microbiota in humans is exposed to various factors, such as foods and medicines in daily life. Because antibiotics, as well as non-antibiotic drugs, have been reported to affect gut microbiota, differences in drug exposure between males and females may be one of the reasons for sex differences in the gut microbiota [48].

In a study of a large cohort in the Netherlands, the males were more likely to be taking drugs for heart disease, whereas the females were more exposed to opiates, laxatives, and antibiotics. Interestingly, the gut microbiota in the females had more antibiotic resistance genes than that in the males [19]. Although it was not consistent with microbial strains showing a sex difference in the entire cohort, drugs that affected the sex hormones altered the gut microbiota. For example, anti-androgen-based oral contraceptives were positively associated with B. caccae and unclassified Coprobacillus strains, and oral contraceptives were associated with an increased abundance of Rothia mucilaginosa [19].

3. Diet

Diet is a most potent modulator of the gut microbiota composition, and animal studies have shown that the interactions between the diet and gut microbiota tended to be sex-dependent, as was observed across different vertebrates, such as wild and laboratory fish, laboratory mice, and humans [8, 49]. The interaction among sex, diet, and gut microbiota also differed between species. It was significant in fish, but not substantial in humans, although there seemed to be a consistent trend that gut microbiota of males was more affected by diet.

The hormonal status affected the microbiota composition more in male mice on a chow diet, whereas this effect was more prevalent in response to a high-fat diet in females [8]. However, the differences between sham control and gonadectomy-treated mice in both sexes were mostly strain-specific. The effect of prebiotics was also different between the sexes. The administration of oligofructose increased the abundance of Bacteroidetes in female rats, but it did not change the microbiota composition in males, even though the butyrate levels were increased [50].

The enterotypes according to the consumed food in humans was reported; however, this study overlooked the differences according to sex [51]. When the existing data were re-analyzed, the correlation of sex and diet affected the abundance of the 125 most common operational taxonomic units, especially those of Fusobacteriaceae [49]. In another study, sex did not have a significant association with the enterotype, but was highly correlated with functional modules, such as enriched aspartate biosynthesis modules in males and enriched inosine monophosphate biosynthesis in females [33].

The underlying mechanism of the sex-dependent interaction between the diet and gut microbiota is still unclear. Fiber intake was related to the gut microbiota composition by reducing the gut transit and pH. In addition, dietary fiber is the main fermentation source used by gut microbiota but has a sex-specific effect [13]. For example, fiber can affect systemic estrogen levels, thereby changing the gut microbiota [52]. Interestingly, there were sex-specific differences in bile acids response to diet. Several bile acids were significantly increased in response to chow diet in gonadectomytreated mice compare to sham-treated mice in both sexes. However, the levels of bile acids in response to a high-fat/high-sucrose diet in gonadectomy-treated male mice is significantly increased compared to that in gonadectomy-treated female mice [8].

4. Body mass index

Although there exist conflicting results, changes in the body weight were associated with alterations in the proportions of Firmicutes and Bacteroidetes in animal and human studies [53, 54]. The relationship between the BMI and sex differences in gut microbiota has been reported in Western and Eastern studies. In an USA study, the relationship of the BMI with the overall gut microbiota composition was significant in the females, but not in the males [13]. In the females, lower diversity indices and a lower Bacteroidetes abundance were observed in the overweight and obese subjects relative to the levels in the normal-weight subjects. In a Spanish study, the Firmicutes/Bacteroidetes ratio of the males was higher in the BMI≤33 kg/m2 group and lower in the BMI>33 kg/m2 group compared with that in the females [15]. At the genus level, the abundance of Bacteroides was significantly higher in the females when the BMI was >33 kg/m2 because the abundance of Bacteroides did not change in the females, whereas it decreased in the males with an increase of the BMI [15]. In this study, the microbiota explained 31.17% of the variation in BMI.

In a Chinese study, the α-diversity of gut microbiota was significantly higher in an underweight group than in the other BMI groups, but in the females only. The relative abundance of Fusobacteria was higher in the obese males, whereas that of Actinobacteria was higher in the obese females [17]. At the genus level, the abundance of Bifidobacterium, Coprococcus, and Dialister was higher and that of Phascolarctobacterium was lower in the obese females, whereas the abundance of Fusobacterium was higher in the obese males.

The total body fat content also seemed to influence the diversity and composition of gut microbiota [17, 55]. Adipose tissue is the main site of extraglandular estrogen synthesis by aromatization of androgens to estrogens, especially in the postmenopausal female [56]. Therefore, it may play an additional role in forming a sex-specific gut microbiota composition.

5. Colonic transit time

The colonic transit time is an important factor determining the gut microbiota composition and metabolism. An in vitro study showed that a prolonged transit time decreased the biomass and diversity in the more distal parts of the gut [57]. The stool consistency is strongly correlated with transit time [58]. In patients with constipation, the profile of the fecal microbiota was associated with colonic transit, and genera from Firmicutes (Faecalibacterium, Lactococcus, and Roseburia) correlated with faster colonic transit [59]. The looser stool consistency, which means the faster colonic transit, the lower the richness and the greater the Prevotella enterotype. Conversely, the harder stool consistency, which means the slower colonic transit, the more Ruminococcaceae-Bacteroides enterotype and higher abundance of Methanobrevibacter and Akkermansia populations [58]. There are differences in bowel function and transit between male and female [60, 61]. In general, females have slower colonic transit time and harder stool form than males [18, 61, 62, 63], but there are some reports that it is not different between the sexes [64]. The colonic transit time was shown to be associated with the level of physical activity, where females showed a more significant interaction in this regard [63]. Although not a consistent finding, females sex is a predisposing factor for producing methane gas, which was shown to delay GI motility in an animal model [65]. Taken together, the studies imply that the different colonic transit times between males and females may contribute to sex differences in the gut microbiota.


Sex differences in the development and presentation of various diseases have been known, but the related mechanism is unclear [66]. Sex differences in the gut microbiota may play a role in the sex differences in diseases.

Irritable bowel syndrome (IBS) is a representative disease with sex difference, occurring twice as often in females [67]. In particular, the proportion of females is higher for patients with IBS suffering severe symptoms who visit a tertiary center. The risk of IBS is increased after infectious colitis, and symptoms begin after infectious colitis in about 10% of patients with IBS (so-called post-infectious IBS) [68]. Interestingly, the incidence of post-infectious IBS is higher in females, suggesting that difference in gut microbiota coming between sexes may play an important role in the pathogenesis of post-infectious IBS [68].

The sex differences in the innate and adaptive immune systems are well known [69]. Receptors for sex hormones are expressed on most immune cells, and thus sex hormones may play a role in establishing the sex difference in the immune response [70]. Because the gut microbiota interacts with the host immune system, it can be assumed that the sex differences in gut microbiota have some role in the sex differences in immunity [40]. NOD mice display spontaneous, immune-mediated destruction of their pancreatic beta cells, resulting in type 1 diabetes mellitus. A higher incidence of diabetes mellitus has been reported in female SPF NOD mice than that of males. Interestingly, this sex difference was not observed in GF NOD mice, whereas it appeared again after the colonization of gut microbiota that are known to be associated with the sex difference in SPF NOD mice [6]. In a study of patients with encephalomyelitis/chronic fatigue syndrome, another example of an immune-related disorder, there was no difference in the overall microbiota composition between the sexes. However, the relative abundance Clostridium, Lactobacillus, Streptococcus, and Bifidobacterium according to specific symptom are different between sexes [71]. Intestinal inflammation might also have sex differences in relation to the gut microbiota. In a mouse model of colitis induced with 2,4,6-trinitrobenzenesulfonic acid, the males exhibited more severe colonic inflammation [72]. The FMT animal model of another study showed that female recipients lost significantly more weight after receiving the male microbiota compared with the weight after receiving the female microbiota, suggesting that the male microbiota caused more gut inflammation [40].

Probiotics also elicited different inflammatory responses from female and male mice [73]. In female Wistar rats exposed to water avoidance stress, the administration of Lactobacillus farciminis significantly lowered the colonic mucosal mast cell count and decreased the levels of inflammatory cytokines only in the female rats [73]. In addition, sex differences in response to probiotic Lactobacillus animalis NP-51 administration were reported for cytokine responses, intestinal metabolic profiles and gut microbiota in Mycobacterium-treated mice [74].


The magnitude of the contribution of sex to the gut microbiota is not so clear when compared with other factors such as diet and medication. Nevertheless, the effect of sex difference on the gut microbiota and its interactions with other factors should be routinely analyzed. Moreover, the result should be stated even if there is no main effect of sex in the study. In the past, some drug side effects that occurred preferentially in females were not discovered until after use of the drug in clinical practice, because clinical studies had been mainly performed using male subjects [24]. Similarly, if the sex is not taken into consideration in studies on the gut microbiota, the sex-dependent effect may be overlooked when a microbiota-based therapeutic strategy is available in clinical practice.

There are several points to consider about the studies that have investigated sex differences in the gut microbiota. Sex differences in the gut microbiota composition have been observed mainly in animal studies. It should be also noted that mice studies have shown that the genetic background is a stronger determinant in shaping intestinal microbiota compared to sex difference [20]. Unlike the case with experimental animals, it is difficult to obtain meaningful results on gut microbiota in a human study, because the sex difference might not be easily noticeable and there are many confounding factors, as we have described above [10, 70].

Another issue is the resolution of currently available methods to investigate gut microbiota. The sex differences in gut microbiota have been observed mainly at the lower taxonomic level; thus current short-read next-generation sequencing (NGS)-based method may have limitations in capturing sex-dependent bacteria [75]. For example, microbes within the same species may produce different metabolites, thus interact differently with sex hormones. Therefore, the full length of 16S rRNA gene or whole genome shotgun sequencing approach may be required to capture the effects of sex differences on gut microbiota, and reveals novel interactions between gut microbiota and sex hormones.

In the future, studies to find the causal relationship among sex, the microbiota, and disease are crucial. To do so, investigations at a finer taxonomic level coupled with multi-omic techniques such as transcriptomics, proteomics, and metabolomics are needed. Not to mention that such studies should be designed to exclude confounding factors as much as possible. These would lead us to treatment strategies that are more tailored to the specific sex for various diseases associated with the gut microbiota.


Disclosure:The authors have no potential conflicts of interest to disclose.

Author Contributions:

  • Conceptualization: YSK.

  • Data curation: YSK, MSP.

  • Formal analysis: TU, YSK, MSP.

  • Funding acquisition: YSK, TU.

  • Investigation: YSK, BYK.

  • Project administration: YSK.

  • Writing – original draft: YSK.

  • Writing – review & editing: YSK, TU, BYK.


The authors thank Professor Moon Young Lee, Department of Physiology, School of Medicine, Wonkwang University for inspiring the creation of this review.

This research was supported by Support Program for Women in Science, Engineering and Technology through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2019H1C3A1032224).

This research was supported, in part, by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A1A03012862).

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